Mouse 8 oxo2deoxyguanosine 8ohdg elisa kit can measure mouse 8 oxo2deoxyguanosine in serum, blood, plasma, cell culture supernatant and other related supernatants and tissues. The micro elisa plate provided in this kit has been precoated with 8ohdg. Mouse 8oxo2deoxyguanosine 8ohdg elisa kit elisa genie. Cited sample types include plasma, seminal fluids, cell. During the reaction,8ohdg in the sample or standard competes with a fixed amount of 8ohdg on the solid phase supporter for sites on the biotinylated detection antibody specific to 8ohdg. Add 100 ul of diluted antigen to appropriate wells. We have developed an easytouse elisa for the detection of the most common marker of rna damage. Nonspecies specific 8ohdg elisa kit colorimetric nbp2. The 8 ohdg monoclonal antibody and the sample or standard are added to a microtiter plate well which has been precoated with 8 ohdg. The 8ohdg 8 hydroxydeoxyguanosine elisa kit accurately measures natural 8 ohdg 8 hydroxydeoxyguanosine levels. Agriseras 8ohdg elisa is a competitive assay that can be used for the. General 8hydroxydeoxyguanosine elisa kit 8 ohdg rk00610 suitable for serum, plasma, cell culture supernatants and urine in general. Detection of other special sample please contact our technical support. Followings are technical tips to have the best results using this product.
The 8ohdg assay preparation reagent set contains nuclease, bu. The concentration of 8ohdg in the samples is determined by comparing the o. During the reaction, 8 ohdg in the sample or standard competes with a fixed amount of 8 ohdg on the solid phase supporter for sites on the biotinylated detection antibody specific to 8 ohdg. Therefore, higher concentrations of 8 ohdg in the sample solution lead to a.
The epiquik 8ohdg dna damage quantification direct kit colorimetric contains all reagents necessary for the quantification of oxidative dna damage 8ohdg. For the measurement of dna damage 8 ohdg in serum, saliva, and urine from any species. Sample or calibrator 8ohdg competes with platebound 8ohdg for binding with the antibody. Protocol summary remove appropriate number of 8ohdg precoated well strips. Originally described by engvall and perlmann 1971, the method. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of dna fragments and free 8 ohdg, concentrations of 8 ohdg reported by elisa methodology will not coincide. Elisa technical guide and protocols thermo fisher scientific. The nwlss 8ohdg elisa assay uses a competitive format wherein a murine monoclonal antibody to 8ohdg primary anti8ohdg and sample or standard are added to a microtiter plate which has been precoated with 8ohdg. Caymans dnarna oxidative damage high sensitivity elisa kit is a competitive assay that can be used to measure 8 ohdg, 8 ohg, and 8 hydroxyguanine. Concentrations of 8oxodg within a cell are a measurement of oxidative stress. Human 8hydroxy2 deoxyguanosine8 ohdgelisa kit this product is suitable for in vitro quantitative detection of human serum, plasma or cell culture supernatant and organizations in the natural and recombinant 8ohdg concentration. The 8ohdg in the sample or standard competes with the 8. The elisa utilizes an 8hydroxy2deoxy guanosinecoated plate and an hrp. During enzyme immunoassay, there should be multiple pores when testing mouse 8.
The elisa utilizes an 8hydroxy2deoxyguanosinecoated plate and an. Each 8 ohdg sample including unknown and standard should be assayed in duplicate. The 8hydroxy2deoxyguanosine 8ohdg elisa assay kit is for research use only and not to be used in diagnostic procedures. The 8 ohdg in the sample or standard competes with the 8 ohdg bound on the plate for the 8 ohdg monoclonal antibody binding sites. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of dna fragments and free 8 ohdg, concentrations of 8 ohdg reported by elisa methodology will not coincide with those. Incubate 2 hours at room temperature or 4 c overnight. The concentration of 8 ohdg in the samples is determined by comparing the o. Urinary 8ohdg measurement using elisa rather than others, such as high performance liquid chromatography hplc, saves analytical time, running costs, and.
Each kit contains sufficient reagents and standards for the replicate quantification of 8 ohdg in up to 18 samples. This elisa kit uses competitiveelisa as the method. We provide two types of 8ohdg elisa kits with different assay range. New 8ohdg check elisa kit is designed to be used easily. Since 8hydroxyguanosine is a modified base which occurs in dna as a result of. This product is a 8ohdg elisa kit utilizing anti 8ohdg monoclonal antibody clone n45. Agriseras 8ohdg elisa is a competitive assay that can be used for the quantification of 8ohdg in urine, cell culture, plasma, and other sample matrices. Enzymelinked immunosorbent assay elisa is widely used to detect 8ohdg in urine or other biological samples with limit of detection lod of one 8ohdgper 10 5 deoxyguanosine dg.
Among numerous types of oxidative dna damage, 8hydroxydeoxyguanosine 8ohdg is a ubiquitous marker of oxidative stress. Elisa is commonly used for the detection of urinary 8oxo7,8dihydro2deoxyguanosine 8oxodg, a marker of whole body oxidative stress. Every product we sell is backed by novus 100% guarantee. Elisa for experiment should be strictly operated according to manual standard and fully preheated beforehand. Pdf urinary 8oxo7,8dihydro2deoxyguanosine analysis. Dilute the proper amount of 8ohdg conjugate 1 mgml to 1. The oxiselect oxidative dna damage elisa kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8ohdg in urine. This elisa kit uses the competitiveelisa principle. Dilute the antigen to 12 ugml in coating solution 2. It is important to note that the 8 ohdg antibody used in this assay recognizes both free 8 ohdg and dnaincorporated 8 ohdg. The nwlss 8ohdg elisa assay uses a competitive format wherein a murine monoclonal antibody to 8ohdg primary anti8ohdg and sample or standard are added to a microtiter plate which has been. Detection of 8oxodg in calf thymus dna using the ht 8oxodg elisa.
Pdf urinary 8oxo7,8dihydro2deoxyguanosine analysis by. It is important to note that the 8ohdg antibody used in this assay recognizes both free 8ohdg and. During enzyme immunoassay, there should be multiple pores when testing mouse 8ohdg standard samples 9. Stressmarqs 8ohdg elisa is a competitive assay that can be used for the. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8ohdg, the value obtained with our assay will be higher than that. Dilute the proper amount of 8 ohdg conjugate 1 mgml to 1. Originally described by engvall and perlmann 1971, the method enables analysis of protein samples immobilized in microplate wells using specific antibodies. This competitive elisa kits employs a monoclonal antibody specific for 8 ohdg quantification in tissue, serum, plasma or urine samples. Depending on the protein of interest, antibodies, buffers, or substrates being used, this general protocol may need to be optimized. In a metaanalysis, an enzymelinked immunosorbent assay elisabased method was used to measure the 8ohdg concentration in leukocyte dna of 314 patients with. Dna damage elisa kit manual immunochemistry technologies. The dna damage competitive elisa researchuseonly kit detects all three oxidized guanine species. Protocol summary remove appropriate number of 8 ohdg precoated well strips.
With automated equipment the assay is easily scalable for set up in highthroughput and can handle a large number of samples in parallel, making it a popular. It is considered as a critical biomarker of oxidative stress and carcinogenesis. Please read the instruction manual enclosed in the product package. The 8ohdg monoclonal antibody and the sample or standard are added to a microtiter plate well which has been precoated with 8ohdg. However, the method has been criticized for high inter. High content 8 ohdg urine or serum samples should be. The cookie settings on this website are set to allow all cookies to give you the very best experience. Stressmarqs 8 ohdg elisa is a competitive assay that can be used for the. Prepare and mix all reagents thoroughly before use. Amphiphysin antibodies and 8ohdg values in blood using the elisa method between asd patients and healthy children to demonstrate the role of neuroin. This kit has been validated for use in urine but other sample types.
Add hrp conjugated 8 ohdg antibody and either standard or sample to appropriate wells. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8ohdg, the value obtained with our assay will be higher than that obtained by competitor elisas or by lc. Recently oxidative rna damage has been described in conjunction with a wide variety of neurological diseases including alzheimers disease, parkinsons disease, dementia with lewy bodies, prion disease, and subacute sclerosing panencephalitis. The sandwich elisa protocol provided here is representative of most readytouse elisa kits for measurement of cytokines, chemokines, growth factors, and other extracellular targets. Mouse 8oxo2deoxyguanosine 8ohdg elisa kit can measure mouse 8oxo2deoxyguanosine in serum, blood, plasma, cell culture supernatant and other related supernatants and tissues. Serum 8 hydroxy2deoxyguanosine level as a potential biomarker of oxidative dna damage induced by ionizing radiation in human peripheral blood yu gao, et al,doseresponse,2019 changes of 8 ohdg and trxr in the residents who bathe in radon hot springs gao yanxiao, et al,doseresponse,2019. During the reaction, 8ohdg in the sample or standard competes with a fixed. Serum 8hydroxy2deoxyguanosine level as a potential biomarker of oxidative dna damage induced by ionizing radiation in human peripheral blood yu gao, et al,doseresponse,2019 changes of 8ohdg. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8 ohdg, the value obtained with our assay will be higher than that obtained by competitor elisas or by lc. Antineuronal antibodies and 8ohdg an indicator of cerebellar. Human 8hydroxy2 deoxyguanosine8 ohdgelisa kit this product is suitable for in vitro quantitative detection of human serum, plasma or cell culture supernatant and organizations in the natural and. The enzymelinked immunosorbent assay elisa is a frequently used application which determines the concentration of an analyte in solution. Antineuronal antibodies and 8ohdg an indicator of cerebellar dysfunction in autism spectrum disorder.
The enzyme linked immunosorbent assay elisa is a powerful method for detecting and quantifying a specific protein in a complex mixture. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8ohdg, the. The anti8ohdg monoclonal antibody and the sample or standard are added to the microtiter plate which has been precoated with 8ohdg. A competitive inhibition reaction is launched between biotin labeled 8hydroxydeoxyguanosine 8ohdg and unlabeled 8hydroxydeoxyguanosine 8ohdg standards or samples with the precoated. Product manual for urinary 8ohdg elisa assay kit northwest life. Oxford biomedicals 8ohdg eia kit is a competitive assay that can be used for the quantification of 8 oh. Agriseras 8 ohdg elisa is a competitive assay that can be used for the. These species may be present in serum, plasma, saliva, urine, dried fecal samples, and tissue culture media samples. This set is able to reduce the variation of the enzyme reaction by addition of each reagent to the sample dna in accordance with the protocol. Ht 8oxodg elisa kits the ht 8oxodg elisas are platebased immunoassays designed for detecting and quantifying 8hydroxy2deoxyguanosine 8oxodg.
Elisa is commonly used for the detection of urinary 8 oxo7, 8 dihydro2deoxyguanosine 8 oxodg, a marker of whole body oxidative stress. This kit is a competitive enzymelinked immunosorbent assay elisa for quantitative measurement of. For the measurement of dna damage 8ohdg in serum, saliva, and urine from any species. Mbs267161 human 8hydroxy2deoxyguanosine8ohdgelisa kit. Caymans dnarna oxidative damage high sensitivity elisa kit is a competitive assay that can be used to measure 8ohdg, 8ohg, and 8hydroxyguanine. Our oxiselect oxidative dna damage elisa kit 8hydroxydeoxyguanosine assay provides a powerful method for rapid, sensitive quantitation of 8ohdg in dna samples.
The elisa utilizes an 8 hydroxy2deoxyguanosinecoated plate and an hrpconjugated antibody for detection which allows for an assay range of 0. General 8hydroxydeoxyguanosine elisa kit 8ohdggeneral. Prepare all the reagents, standards and samples as instructed. Novus biologicals 8ohdg elisa is a competitive assay that can be used for. Dnarna oxidative damage high sensitivity elisa kit cayman. Human 8hydroxydeoxyguanosine 8ohdg elisa kit cusabio. Put the unused microplates into raw foil bag at 28. Standards, test samples, and biotinconjugated reagent are added to the wells and incubated. There are currently no images for 8 ohdg elisa kit nbp262143. Calf thymus dna was untreated or treated with increasing concentrations of chromium iii chloride crcl 3, in the presence of 0. Kit components and assay qc data details of dna damage 8ohdg elisa kit the quality control qc data in this section is obtained from bosters internal qc results and is for reference only. In this assay, dna is bound to strip wells that are specifically treated to have a high dna affinity. Since 8 hydroxyguanosine is a modified base which occurs in dna as a result of oxidative stress, hence considered a sensitive marker of oxidative damage in cellular dna, its structure is pretty conserved among different species. This package insert must be read in its entirety before using.